Canine Distemper Virus Antigen CDV AG Rapid Test Kit Result Reading Time 10-15 Mins

【INTENDED USE】

The Canine Distemper Virus (CDV) Antigen Rapid Test Kit is a chromatographic immunoassay for the qualitative detection of Canine Distemper Virus antigen in canine eye, ocular or nasal secretion.

【PRINCIPLE】

The invisible anti-CDV antibody(T line) and secondary antibody (C line) are immobilized on a nitrocellulose membrane in result window.Another anti-CDV antibody is conjugated with colloidal gold particle and immobilized on glass fiber as conjugate pad.When specimen is applied into the sample well on device,CDV antigen will bind to the gold conjugate and migrate on the surface of test strip by passive diffusion,and a visible T line will appear in result window after 10 minutes.The C line has to appear every time when the test has performed which indicates the test is performing properly. 【CONTENTS】

• Rapid Test

• Buffer

• Disposable pipettes

• Swab

• Instruction for use

【STORAGE AND STABILITY】

• The kit should be stored at 2-30°C until the expiry date printed on the sealed pouch.

• The test must remain in the sealed pouch until use.

• Keep away from direct sunlight, moisture and heat.

• Do not freeze.

• Care should be taken to protect the components of the kit from contamination. Do not use if there is evidence of microbial contamination or precipitation. Biological contamination of dispensing equipment, containers or reagents can lead to false results.

【PRECAUTIONS】

• The test kit is for canine use only. Do not use for other animals.

• The device is sensitive to humidity and heat.Perform the test immediately after removing it from the foil pouch.

• Read the instructions carefully before operation and follow the instructions..

• Do not reuse the test components

• Do not use the test kit if the pouch is damaged or the sea

is broken.

• Do not use the test kit beyond the stated expiration date marked on the label.

• Do not mix components from different lot numbers because the components in this kit have been quality control tested as standard batch unit.

• All specimens should be handled as being potentially infectious.

• Decontaminate and dispose of all specimens, used kits and potentially contaminated materials safely in accordance with national and local regulations.

【SAMPLE COLLECTION AND PREPAIRATION】

• Specimen of this test is canine body secretions of eye mucus (in the conjunctiva) or nasal discharge.

• The specimens should be tested immediately after collection.

• If specimens are not tested immediately, they should be stored at 2~8°C for 24 hours. For longer storage, freeze at -20°C or below

【OPERATION】

Bring tests, specimens, buffer and/or controls to room temperature (15- 30°C) before use.

1. Collect canine ocular or nasal secretion using a swab, then insert the swab into the assay buffer tube.

2. Mix the swab until the specimen has been dissolved totally into the assay buffer (Appx. 10s).

3. Remove the test from its sealed pouch, and place it on a clean, level surface. Label the cassette device with animal patient or control identification.

4. Transfer 3 drops of mixing buffer into the sample well on device, drop by drop vertically, and start the timer.

5. Wait for the colored band(s) to appear. The result should be read at 15 minutes. Do not interpret the result after 20 minutes.

【INTERPRETATION OF RESULTS】

POSITIVE: The presence of two lines as control line (C) and test line (T) within the result window indicates a positive result.

NEGATIVE:The presence of only control line (C) withintheresultwindow indicates a negative result.

INVALID: If the control line (C) is not visible withintheresultwindow after performing the test, the result isconsideredinvalid. Some causes of invalid results are becauseofnotfollowing the directions correctly or the test mayhavedeteriorated beyond the expiration date. It is recommendedthat the specimen be re-tested using a newtest.

【TEST LIMITATIONS】

• This kit can provide fast and easy way toget aresult,butdo not completely exclude the possibilityofalowincidence of false results which occur byvariousfactors,veterinarians should also consider otherclinicalsymptoms and laboratory diagnostic methods tomakethedefinite diagnosis.

• The kit will only indicate the presenceofcaninedistemper virus in the specimen or not.

• A negative result may be obtained if the concentrationofthe canine distemper virus present in the specimenisnotadequate or is below the detectable level of the test.

For further operation or performance details, please refer to final instruction manual.