Manual and Auto Nasopharyngeal Swab Nasal Sample Magnetic Bead Nucleic Acid Extraction Kit FDA

RNA Manual and Auto Extraction Nucleic Acid Extraction Kit ( Magnetic Bead Method )

【Package of RNA Isolation Kit】

48 preps, 96 preps

【Application of RNA Isolation Kit】

It is used for nucleic acids isolation, enrichment and purification.The processed products can be used for clinical in vitro diagnosis.

【Main Components of RNA Isolation Kit】

【Principle of RNA Isolation Kit】

This product provides a simple, rapid and efficient method to extract DNA/RNA from sample. The unique “Less reagent by higher accuracy” system enables the nucleic acid in the lysate to be efficiently and specifically binded to the magbeads. The obtained nucleic acid has high purity, stable quality, and is free of protein, nuclease and other contaminants and inhibitors. It can be applied to various conventional operations, including PCR, fluorescence quantitative PCR and other experiments.

【Storage & Shelf Life 】

• 12 months shelf life in Room Temperature Stock 4-30°C before package open.
• 7 days shelf life at - 20°C after extraction.

【Sample Requirements】

Applicable Samples: Whole blood, tissue homogenate, swab, serum, plasma, bronchoalveolar lavage fluid and other cell-free body fluids.

【Applicable Instruments】

1) Manual operation: magnetic separator, centrifuge, dry bath.

2) Nucleic acid extraction system: automatic or semi-automatic nucleic acid extraction system based on magnetic bead absorption principle.

【Sample Requirements】

• Sample types: Liquid samples such as serum, plasma, nasopharyngeal swab, cell preservation fluid , tissue fluid, urine and secretions.
• Sample collection: Collect sample via routine method.
• Sample preservation and transportation: The samples collected can be used for nucleic acid extraction immediately, or stored at -20℃ for long-term preservation. Prevent from freeze-thaw cycles. The samples should be transported in sealed cooler box or styrofoam box with ice seal.

【Procedure】

Things to do before starting: Mix all the reagents and gently invert 3-5 times before use. Shake the bottle containing Magbeads Suspension Solution and vortex for 2 minutes (before first use) or 1 minute (before subsequent use) to ensure that the magbeads are fully resuspended before use.

• Manual extraction

1.1 Break the testing swab into the tube with Preservation solution, seal the tube cover and heat it in 60°C for 20mins.

1.2 Take out the swab head, add 50μL Reaction solution and mix, then add 600μL Magbeads solution, seal the tube cover and gently mix it by mixer for 10-15mins in room temperature.

1.3 Use Magnet to attract the magbeads, pour the supernatant and leave the precipitation in the tube.

1.4 Add 600μL Rinse into tube to wash and mix for 5seconds, then use magnet to attract the magbeads again, repeat this operation for one time, then use vacuum pump to suck away the supernatant. Seal the tube cover and heat it in 100℃ for 1 min or wait till it is dry .

1.5 Add 30~50μL Eluent to wash the nucleic acid from magbeads, heat the tube in 60℃ to accelerate elution. Take the supernatant as template to PCR analysis.

• Automated extraction

Read from the extraction analyzer operation manual.