| Place of Origin: | China |
|---|---|
| Brand Name: | Dewei |
| Certification: | CE, FDA, ISO |
| Model Number: | DW-80007 |
| Minimum Order Quantity: | 5,000 kits |
| Price: | $0.3-$2.0/unit |
| Packaging Details: | 48 preps/kit, 96 preps/kit |
| Delivery Time: | in 2-5 days |
| Payment Terms: | T/T, Western Union, L/C, MoneyGram |
| Supply Ability: | 200,000 units/Day |
| Usage: | Covid-19 RNA Extraction | Sample: | Covid-19 Oral Or Nasal Sample |
|---|---|---|---|
| Temperature: | 2-30℃, No Need Cool Chain | Shelf Life: | 12 Months |
| Component: | Preservation Solution, Reaction Solution, Magbeads Solution, Rinse, Eluent | Method: | Magnetic Bead Method |
| High Light: | ISO Nucleic Acid Extraction Kit,Magnetic Bead Nucleic Acid Extraction Kit,Nasal Sample RNA Extraction Kits |
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RNA Manual Extraction Nucleic Acid Extraction Kit ( Magnetic Bead Method ) Less Step
Advantages: Less Reagents by Less Steps in room temperature stock!
Package
48 preps, 96 preps
Application
It is used for nucleic acids isolation, enrichment and purification.The processed products can be used for clinical in vitro diagnosis.
Main Components
| Component | 48 preps | 96 preps | Single Test Consumption | ||
| SIZE | QTY | SIZE | QTY | ||
| Preservation Solution | 600μL | 48 | 600μL | 96 | 600μL |
| 3ml | 48 | 3ml | 96 | for nasal swab sample | |
| Reaction Solution | 1.5ml | 2 | 6ml | 1 | 50μL |
| Magbeads Solution | 30ml | 1 | 60ml | 1 | 600μL |
| Rinse | 60ml | 1 | 120ml | 1 | |
| Eluent | 1.5ml | 2 | 6ml | 1 | 50μL |
Storage & Shelf Life of Extraction Kit
Sample Requirements of Extraction Kit
Applicable Samples: Whole blood, tissue homogenate, swab, serum, plasma, bronchoalveolar lavage fluid and other cell-free body fluids.
Principle of Extraction Kit
This product provides a simple, rapid and efficient method to extract DNA/RNA from sample. The unique “Less reagent by higher accuracy” system enables the nucleic acid in the lysate to be efficiently and specifically binded to the magbeads. The obtained nucleic acid has high purity, stable quality, and is free of protein, nuclease and other contaminants and inhibitors. It can be applied to various conventional operations, including PCR, fluorescence quantitative PCR and other experiments.
Applicable Instruments
1) Manual operation: magnetic separator, centrifuge, dry bath.
2) Nucleic acid extraction system: automatic or semi-automatic nucleic acid extraction system based on magnetic bead absorption principle.
Procedure
Things to do before starting: Mix all the reagents and gently invert 3-5 times before use. Shake the bottle containing Magbeads Suspension Solution and vortex for 2 minutes (before first use) or 1 minute (before subsequent use) to ensure that the magbeads are fully resuspended before use.
1.1 Break the testing swab into the tube with Preservation solution, seal the tube cover and heat it in 60°C for 20mins.
1.2 Take out the swab head, add 50μL Reaction solution and mix, then add 600μL Magbeads solution, seal the tube cover and gently mix it by mixer for 10-15mins in room temperature.
1.3 Use Magnet to attract the magbeads, pour the supernatant and leave the precipitation in the tube.
1.4 Add 600μL Rinse into tube to wash and mix for 5seconds, then use magnet to attract the magbeads again, repeat this operation for one time, then use vacuum pump to suck away the supernatant. Seal the tube cover and heat it in 100℃ for 1 min or wait till it is dry .
1.5 Add 30~50μL Eluent to wash the nucleic acid from magbeads, heat the tube in 60℃ to accelerate elution. Take the supernatant as template to PCR analysis.
Read from the extraction analyzer operation manual.