Dengue NS1 Antigen Test Card Rapid Test Kit
INTENDED USE
The Dengue Rapid Test Device (Whole Blood/Serum/Plasma) is a rapid chromatographic immunoassay for the qualitative detection of IgG and IgM antibodies to Dengue virus in human whole blood, serum, or plasma as an aid in the diagnosis of primary and secondary Dengue infections.
INTRODUCTION
Dengue is a flavivirus, transmitted by Aedes aegypti and Aedes albopictus mosquitoes. It is widely distributed throughout the tropical and subtropical areas of the world,1 and causes up to 100 million infections annually. Classic Dengue infection is characterized by a sudden onset of fever, intense headache, myalgia, arthralgia and rash. Primary Dengue infection causes IgM antibodies to increase to a detectable level in 3 to 5 days after the onset of fever. IgM antibodies generally persist for 30 to 90 days. Most Dengue patients in endemic regions have secondary infections, resulting in high levels of specific IgG antibodies prior to or simultaneous with IgM response. Therefore, the detection of specific anti-Dengue IgM and IgG antibodies can also help to distinguish between primary and secondary infections.
The Dengue Rapid Test Device (Whole Blood/Serum/Plasma) is a rapid test that utilizes a combination of Dengue antigen coated colored particles for the detection of IgG and IgM Dengue antibodies in human whole blood, serum, or plasma.
MAIN CONTENTS
• One pouch containing a reaction test Cassette with desiccant.
• Buffer
• Disposable pipettes.
• Instructions for use.
STORAGE AND STABILITY
• Store at 39~ 86 º F (4 ~ 30 º C) in the sealed pouch for 18 months.
PRECAUTIONS
• For in vitro diagnostic use only.
• Do not use after expiration date.
• The test Cassette should remain in the sealed pouch until use.
• The used test Cassette should be discarded according to local regulations.
DIRECTION OF USE
Allow the test device, specimen, buffer, and/or controls to reach room temperature (15 30°C) prior to testing.
1. Bring the pouch to room temperature before opening. Remove the test device from the sealed pouch and use it as soon as possible.
2. Place the test device on a clean and level surface.
For Serum or Plasma Specimens:
Hold the dropper vertically, draw the specimen up to the Fill Line (approximately 5 uL), and transfer the specimen to the specimen well (S) of the test device, then add 3 drops of buffer (approximately 90 mL) and start the timer. See illustration below. Avoid trapping air bubbles in the specimen well (S).
For Whole Blood (Venipuncture/Fingerstick) Specimens:
To use a dropper: Hold the dropper vertically, draw the specimen 0.5-1 cm above the Fill Line, and transfer 1 drop of whole blood (approximately 10 µL) to the specimen well (S) of the test device, then add 3 drops of buffer (approximately 90 uL) and start the timer. See illustration below.
To use a micropipette: Pipette and dispense 10 µL of whole blood to the specimen well (S) of the test device, then add 3 drops of buffer (approximately 90 µL) and start the timer. See illustration below.
3. Wait for the colored line(s) to appear. Read results at 10 minutes. Do not interpret the result after 20 minutes.
INTERPRETATION
POSITIVE: Two colored bands appear on the membrane. One band appears in the control region (C) and another band appears in the test region (T).
NEGATIVE: Only one colored band appears, in the control region (C). No apparent colored band appears in the test region (T).
INVALID: Control band fails to appear. Results from any test which has not produced a control band at the specified read time must be discarded. Please review the procedure and repeat with a new test. If the problem persists, discontinue using the kit immediately and contact your local distributor.
FRQ:
| 1 |
What are 7 warning signs of dengue? |
Warning Signs*
Abdominal pain or tenderness.
Persistent vomiting.
Clinical fluid accumulation.
Mucosal bleed.
Lethargy or restlessness.
Liver enlargement > 2 cm.
Laboratory finding of increasing HCT concurrent with rapid decrease in platelet count. |
| 2 |
How long will dengue fever last? |
How Long Does Dengue Fever Last? Symptoms can start anywhere from 4 days to 2 weeks after being bitten by an infected mosquito, and typically last for 2 to 7 days. |
| 3 |
What is the best treatment for dengue? |
Most cases of dengue fever can be treated at home with pain medicine. Acetaminophenparacetamol is often used to control pain. Non-steroidal anti-inflammatory drugs like ibuprofen and aspirin are avoided as they can increase the risk of bleeding. For people with severe dengue, hospitalization is often needed. |
| 4 |
Is dengue very serious? |
Severe dengue fever can cause internal bleeding and organ damage. Blood pressure can drop to dangerous levels, causing shock. In some cases, severe dengue fever can lead to death. Women who get dengue fever during pregnancy may be able to spread the virus to the baby during childbirth. |
| 5 |
How do you confirm dengue? |
If an infection is suspected, you will get a blood test to check for the dengue virus. During a blood test, a health care professional will take a blood sample from a vein in your arm, using a small needle. After the needle is inserted, a small amount of blood will be collected into a test tube or vial. |
| 6 |
What not to eat in dengue? |
It is best to choose a lighter diet and avoid oily and fried food. It contains a lot of fat which may lead to higher blood pressure. This can hamper your recovery as it weakens the immune system. |
| 7 |
What is the danger period of dengue? |
Dengue in the critical phase occurs from the 4th to the 7th day since the patient shows symptoms of fever. At this time, the patient may not have a fever or reduce his fever, so he will appear subjective and do not go to the hospital for treatment, making the disease more dangerous. |
| 8 |
Can dengue recover by itself? |
Dengue fever usually lasts between 2 to 7 days.
Most people recover from dengue infection without requiring hospital admission. |
| 9 |
What kills dengue virus? |
To fight the infection, the immune system produces antibodies to neutralize the dengue viral particles, and the complement system is activated to help the antibodies and white blood cells remove the virus. The immune response also includes cytotoxic T cells (lymphocytes), which recognize and kill infected cells. |
| 10 |
Which antibiotic is best for dengue? |
There is no specific medicine to treat dengue infection. If you think you may have dengue fever, you should use pain relievers with acetaminophenand avoid medicines with aspirin, which could worsen bleeding. You should also rest, drink plenty of fluids, and see your doctor. |
| 11 |
How to recover fast from dengue? |
Turmeric with milk is best remedy for faster recovery. Citrus fruits play an important role for dengue patients and it help in recovery. So, rich mix of antioxidants and vitamin C like orange, strawberries, lemon, papaya etc should be included in a diet twice a day," says Dr Sharma. |
| 12 |
Is a CBC test for dengue? |
Complete Blood Count (CBC): The complete blood count test is employed to assess various components of the blood. One crucial aspect is the platelet count, which tends to decrease during the later stages of dengue fever. Monitoring platelet levels is essential as low platelet count can lead to bleeding complications. |
| 13 |
How many days do platelets fall in dengue? |
Platelet counts usually drop significantly by day 4 of illness. In adults without dengue shock, platelet counts drop slightly to moderate from day 3 to day 7 of illness and return to normal by day 8 or 9. |
| 14 |
What is the recovery stage of dengue? |
The recovery phase is the final stage of dengue fever. Individuals who have passed the febrile phase without entering the critical phase, or those who have recovered from the critical second phase for 1-2 days, will reach the recovery phase. |
| 15 |
Can I test dengue at home? |
These tests look for the NS1 protein from the dengue virus in the blood. They usually use special antibodies with labels to find this protein. Orange Health Labs offers the convenience of dengue fever NS1 Antigen Rapid Test at home anywhere in Bangalore. |
| 16 |
Can I drink milk in dengue? |
You can drink more milk to enhance nutrition. Do not eat rice or hard foods that require a lot of chewing and are difficult to swallow. Especially, for children with dengue fever, milk is the main and most important source of nutrition. |
| 17 |
What to not eat in dengue? |
Dengue patients must exclude raw & strongly flavored veggies from their diet, such as cabbage, capsicum, turnip, radish, onion, garlic, eggplant, etc. |
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