CE Dengue Antigen DEN NS1 Rapid Test Kit Cassette For Whole Blood Serum Plasma
【INTENDED USE】
The Dengue NS1 Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid
chromatographic immunoassay for the qualitative detection of NS1 antigen of Dengue
virus in human whole blood, serum, or plasma as an aid in the diagnosis of Dengue
infections.
【INTRODUCTION】
The Dengue NS1 Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid test that
utilizes a combination of Dengue antibodies coated colored particles for the detection of
Dengue NS1 antigen in human whole blood, serum, or plasma.
【SPECIMEN COLLECTION AND PREPARATION】
The Dengue NS1 Rapid Test Cassette (Whole Blood/Serum/Plasma) can be performed using whole blood, serum, or plasma.
To collect Fingerstick Whole Blood specimens:
Wash the patient’s hand with soap and warm water or clean with an alcohol swab.
Allow to dry.
- Massage the hand without touching the puncture site by rubbing down the hand towards the fingertip of the middle or ring finger.
- Puncture the skin with a sterile lancet. Wipe away the first sign of blood.
- Gently rub the hand from wrist to palm to finger to form a rounded drop of blood over the puncture site.
- Add the Fingerstick Whole Blood specimen to the test by using a capillary tube: 1,Touch the end of the capillary tube to the blood until filled to approximately 75uL. Avoid air bubbles. 2,Place the bulb onto the top end of the capillary tube, then squeeze the bulb to dispense the whole blood to the specimen area of the test cassette.
- Add the Fingerstick Whole Blood specimen to the test by using hanging drops: Position the patient’s finger so that the drop of blood is just above the specimen area of the test cassette.
- Allow 3 hanging drops of fingerstick whole blood to fall into the center of the specimen area on the test cassette, or move the patient’s finger so that the hanging drop touches the center of the specimen area. Avoid touching the finger directly to the specimen area.
- Separate serum or plasma from blood as soon as possible to avoid hemolysis. Use only clear, non-hemolyzed specimens.
- Testing should be performed immediately after specimen collection. Do not leave the specimens at room temperature for prolonged periods. Serum and plasma specimens may be stored at 2-8°C for up to 3 days. For long-term storage, specimens should be kept below -20°C.
- Whole blood collected by venipuncture should be stored at 2-8°C if the test is to be run within 2 days of collection. Do not freeze whole blood specimens.Whole blood collected by fingerstick should be tested immediately.
- Bring specimens to room temperature prior to testing. Frozen specimens must be completely thawed and mixed well prior to testing. Specimens should not be frozenand thawed repeatedly.
- If specimens are to be shipped, they should be packed in compliance with federal regulations for transportation of etiologic agents.
【MATERIALS 】
- Materials provided
- Test cassettes
- Droppers
- Buffer
- Package insert
Materials required but not provided
- Specimen collection containers
- Centrifuge (for plasma only)
- Micropipette
- Timer
【DIRECTIONS FOR USE】
Allow the test, specimen, buffer and/or controls to reach room temperature (15-30°C)
prior to testing.
1. Bring the pouch to room temperature before opening it. Remove the test cassette from
the sealed pouch and use it within 1 hour.
2. Place the cassette on a clean and level surface.
For Serum or Plasma specimen:
Hold the dropper vertically and transfer 3 drops of serum or plasma (approximately 75μL) to the specimen area, and start the timer. See illustration below.
For Venipuncture Whole Blood specimen:
Hold the dropper vertically and transfer 3 drops of whole blood (approximately 75 μL) to the specimen area, then add 1 drops of buffer (approximately 40 μL), and start the timer. See illustration below.
For Fingerstick Whole Blood specimen:
To use a capillary tube:
Fill the capillary tube and transfer approximately 75 μL of fingerstick whole blood specimen to the specimen area of test cassette, then add 1 drop of buffer (approximately 40 μL) and start the timer. See illustration below.
To use hanging drops:
Allow 3 hanging drops of fingerstick whole blood specimen
(approximately 75 μL) to fall into the specimen area of test cassette, then add 1 drop of buffer (approximately 40 μL) and start the timer. See illustration below.
3. Wait for the colored line(s) to appear. Read results at 10 minutes. Do not interpret the
result after 20 minutes.
【INTERPRETATION OF RESULTS】
(Please refer to the illustration above)
POSITIVE: * Two distinct colored lines appear. One color line should be in the control region (C) and another color line should be in the test region (T).
*NOTE: The intensity of the color in the test line region (T) will vary depending on the concentration of Dengue NS1 antigen present in the specimen. Therefore, any shade of red in the test region should be considered positive.
NEGATIVE: One color line appears in the control region (C). No apparent red or pink line appears in the test region (T).
INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test cassette. If the problem persists, discontinue using the test kit immediately and contact your local distributor.
【Manufactured by】
DEWEI MEDICAL EQUIPMENT CO.,LTD
5th floor, No. 4 building, Shiyou Industrial Park, Jun'an , Shunde, Foshan, China 52832
FRQ:
| 1 |
What are 7 warning signs of dengue? |
Warning Signs*
Abdominal pain or tenderness.
Persistent vomiting.
Clinical fluid accumulation.
Mucosal bleed.
Lethargy or restlessness.
Liver enlargement > 2 cm.
Laboratory finding of increasing HCT concurrent with rapid decrease in platelet count. |
| 2 |
How long will dengue fever last? |
How Long Does Dengue Fever Last? Symptoms can start anywhere from 4 days to 2 weeks after being bitten by an infected mosquito, and typically last for 2 to 7 days. |
| 3 |
What is the best treatment for dengue? |
Most cases of dengue fever can be treated at home with pain medicine. Acetaminophenparacetamol is often used to control pain. Non-steroidal anti-inflammatory drugs like ibuprofen and aspirin are avoided as they can increase the risk of bleeding. For people with severe dengue, hospitalization is often needed. |
| 4 |
Is dengue very serious? |
Severe dengue fever can cause internal bleeding and organ damage. Blood pressure can drop to dangerous levels, causing shock. In some cases, severe dengue fever can lead to death. Women who get dengue fever during pregnancy may be able to spread the virus to the baby during childbirth. |
| 5 |
How do you confirm dengue? |
If an infection is suspected, you will get a blood test to check for the dengue virus. During a blood test, a health care professional will take a blood sample from a vein in your arm, using a small needle. After the needle is inserted, a small amount of blood will be collected into a test tube or vial. |
| 6 |
What not to eat in dengue? |
It is best to choose a lighter diet and avoid oily and fried food. It contains a lot of fat which may lead to higher blood pressure. This can hamper your recovery as it weakens the immune system. |
| 7 |
What is the danger period of dengue? |
Dengue in the critical phase occurs from the 4th to the 7th day since the patient shows symptoms of fever. At this time, the patient may not have a fever or reduce his fever, so he will appear subjective and do not go to the hospital for treatment, making the disease more dangerous. |
| 8 |
Can dengue recover by itself? |
Dengue fever usually lasts between 2 to 7 days.
Most people recover from dengue infection without requiring hospital admission. |
| 9 |
What kills dengue virus? |
To fight the infection, the immune system produces antibodies to neutralize the dengue viral particles, and the complement system is activated to help the antibodies and white blood cells remove the virus. The immune response also includes cytotoxic T cells (lymphocytes), which recognize and kill infected cells. |
| 10 |
Which antibiotic is best for dengue? |
There is no specific medicine to treat dengue infection. If you think you may have dengue fever, you should use pain relievers withacetaminophen and avoid medicines with aspirin, which could worsen bleeding. You should also rest, drink plenty of fluids, and see your doctor. |
| 11 |
How to recover fast from dengue? |
Turmeric with milk is best remedy for faster recovery. Citrus fruits play an important role for dengue patients and it help in recovery. So, rich mix of antioxidants and vitamin C like orange, strawberries, lemon, papaya etc should be included in a diet twice a day," says Dr Sharma. |
| 12 |
Is a CBC test for dengue? |
Complete Blood Count (CBC): The complete blood count test is employed to assess various components of the blood. One crucial aspect is the platelet count, which tends to decrease during the later stages of dengue fever. Monitoring platelet levels is essential as low platelet count can lead to bleeding complications. |
| 13 |
How many days do platelets fall in dengue? |
Platelet counts usually drop significantly by day 4 of illness. In adults without dengue shock, platelet counts drop slightly to moderate from day 3 to day 7 of illness and return to normal by day 8 or 9. |
| 14 |
What is the recovery stage of dengue? |
The recovery phase is the final stage of dengue fever. Individuals who have passed the febrile phase without entering the critical phase, or those who have recovered from the critical second phase for 1-2 days, will reach the recovery phase. |
| 15 |
Can I test dengue at home? |
These tests look for the NS1 protein from the dengue virus in the blood. They usually use special antibodies with labels to find this protein. Orange Health Labs offers the convenience of dengue fever NS1 Antigen Rapid Test at home anywhere in Bangalore. |
| 16 |
Can I drink milk in dengue? |
You can drink more milk to enhance nutrition. Do not eat rice or hard foods that require a lot of chewing and are difficult to swallow. Especially, for children with dengue fever, milk is the main and most important source of nutrition. |
| 17 |
What to not eat in dengue? |
Dengue patients must exclude raw & strongly flavored veggies from their diet, such as cabbage, capsicum, turnip, radish, onion, garlic, eggplant, etc.
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