|Test Parameter:||Rotavirus||Storage:||Room Temperature 2-30℃|
|Main Component:||Rapid Test Cassette + Buffer + Dropper + IFU||Sample:||Feces|
|Reading Time:||In 15 Mins||Test Result:||Qualitative Positive Or Negative|
|Age:||Most Among Kids||Princile:||Immunochromatography|
Rotavirus Cassette Rapid Test,
One Step Cassette Rapid Test
The Rotavirus Rapid Test Device (Feces) is a rapid visual immunoassay for the qualitative presumptive detection of rotavirus in human fecal specimens. This kit is intended to be used as an aid in the diagnosis of rotavirus infection.
Rotavirus is the most common agent responsible for acute gastroenteritis, mainly in young children. Its discovery in 1973 and its association with infantile gastro-enteritis represented a very important advancement in the study of gastro-enteritis not caused by acute bacterial infection. Rotavirus is transmitted by oro-faecal route with an incubation period of 1-3 days. Although specimen collections taken within the second and fifth day of the illness are ideal for antigen detection, the rotavirus may still be found while diarrhoea continues. Rotaviral gastroenteritis may result in mortality for populations at risk such as infants, the elderly, and immunocompromised patients. In temperate climates, rotavirus infections occur mainly in the winter months. Endemics as well as epidemics affecting some thousand people have been reported. With hospitalised children suffering from acute entric disease up to 50% of the analysed specimen were positive for rotavirus. The viruses replicate in the cell nucleus and tend to be host species specific producing a characteristic cytopathic effect (CPE). Because rotavirus is extremely difficult to culture, it is unusual to use isolation of the virus in diagnosing an infection. Instead, a variety of techniques have been developed to detect rotavirus in feces.
Bring tests, specimens, buffer and/or controls to room temperature (15-30°C) before use.
For liquid specimens: Hold the pipette vertically, aspirate fecal specimens, and then transfer 2 drops (approximately 50 µL) into the specimen collection tube containing the extraction buffer.
Avoid trapping air bubbles in the specimen well (S), and do not drop any solution in observation window.
As the test begins to work, you will see color move across the membrane.
Note: If the specimen does not migrate (presence of particles), centrifuge the extracted specimens contained in the extraction buffer vial. Collect 80 µL of supernatant, dispense into the specimen well (S) of a new test device and start afresh following the instructions mentioned above.
Rapid Test Cassette in Pouch
Store as packaged in the sealed pouch at room temperature or refrigerated (2-30°C).
The test is stable through the expiration date printed on the sealed pouch.
The test must remain in the sealed pouch until use.
DO NOT FREEZE.
Do not use beyond the expiration date.
Relative Sensitivity: 99.1% (96.8%-99.9%)*
Relative Specificity: >99.9% (97.7%-100.0%)*
Overall Agreement: 99.5% (98.1%-99.9%)*
*95% Confidence Interval
For details, please contact Dewei staff for Instruction Manual!