Norovirus Antigen Rapid Test Instruction
| Cat# |
Product Name |
Sample |
Method |
Quantity per box |
Certificate |
| NOR-DF02 |
Nonovirus |
Feces |
Lateral Flow |
25tests |
CE, ISO |
Description
This Norovirus Antigen Rapid Test Kit is used for qualitative detection of norovirus antigen (GI) and Norovirus antigen (GII) in human stool.
This kit is coated with goat anti-mouse IgG polyclonal antibody (C) on nitrocellulose membrane using colloidal gold immunochromatography Line), mouse anti-norovirus monoclonal antibody (GII-2) and mouse anti-norovirus monoclonal antibody (GI-2): Colloidal gold labeled mouse anti-norovirus monoclonal antibody (GII-1) and mouse anti-norovirus monoclonal antibody (G1-1) were fixed on gold label pad. When a positive sample is tested, the norovirus antigen (GII) and/or Norovirus antigen (GI) in the sample combine with the mouse anti-norovirus monoclonal antibody (GII-1) and/or mouse anti-norovirus monoclonal antibody (GI-1) on the gold pad to form a complex that is moved along the membrane by chromatography. After passing the test line, it forms a sandwich complex with the pre-coated antibody to coagulate and develop color, and combines with goat anti-mouse IgG polyclonal antibody at the quality control line to develop color, while negative samples only develop color at the quality control line.
Information
Norovirus (NoV), also known as norovirus, belongs to the calciviruses family and is mainly transmitted through contaminated water, food, contact or aerosols formed by pollutants.
The symptoms of Norovirus related diseases are those typical of gastroenteritis, that is, vomiting, watery diarrhea and abdominal cramps. Vomiting is a characteristic symptom in the majority of Norovirus infections (64% pf adults and 81% of children). Other symptoms such as general malaise, low grade fever, nausea and fatigue are also present in over 90% of cases.
Norovirus plays an important role in the outbreak of non-bacterial gastroenteritis, which is associated with 30% to 50% of the aseptic gastroenteritis, and 90% of the viral gastroenteritis caused by food is caused by norovirus ".
Contents
- Rapid Test
- Speciment diluent tube with buffer
- Disposable pipette
- Instruction for use
Direction of Use
1. Specimen collection and pre-treatment:
1) Use the specimen collection cards provided in the kit for specimen collection. Follow the instructions on the specimen collection card. Other clean, dry containers may also be used for specimen collection. For best results, performed the test within 6 hours after collection.
2) Unscrew and remove the dilution tube applicator. Be careful not to spill or spatter solution from the tube. Collect specimens by inserting the applicator stick into at least 3 different sites of the feces.
3) Replace the applicator back into the tube and screw the cap tightly. Be careful not to break the tip of the dilution tube.
4) Shake the specimen collection tube vigorously to mix the specimen and extraction buffer. Specimens prepared in the specimen collection tube may be stored for 6 months at -20°C if not tested within 1 hour after preparation.
2. Testing
1) Remove the test from its sealed pouch, and place it on a clean, level surface. Label the test with patient or control identification. For best results, the assay should be performed within one hour.
2) Using a piece of tissue paper, break the tip of the dilution tube. Hold the tube vertically and dispense 2 drops of solution into the specimen well (S) of the test device.
Avoid trapping air bubbles in the specimen well (S), and do not add any solution to the result area.
As the test begins to work, color will migrate across the membrane.
3. Wait for the colored band(s) to appear. The result should be read at 10 minutes. Do not interpret the result after 20 minutes.
3.Results
1) Positive:
Norovirus GI Positive: * A colored band appears in the control band region (C) and another colored band appears in the GI band region.
Norovirus GⅡ Positive: * A colored band appears in the control band region (C) and another colored band appears in the GII line region.
Norovirus GI and GⅡ Positive: * A colored band appears in the control line region (C) and two other colored bands appear in GI line region and GII line region respectively.
2) Negative:
One colored band appears in the control band region (C). No band appears in the test band region (GI/GII).
3)Invalid:
Control band fails to appear. Results from any test which has not produced a control band at the specified reading time must be discarded.
Detail Picture
Precautions
- For professional in vitro diagnostic use only.
- Do not use after expiration date indicated on the package. Do not use the test if its foil pouch is damaged. Do not reuse tests.
- This kit contains products of animal origin. Certified knowledge of the origin and/or sanitary state of the animals does not totally guarantee the absence of transmissible pathogenic agents. It is therefore, recommended that these products be treated as potentially infectious, and handled observing the usual safety precautions (do not ingest or inhale).
- Avoid cross-contamination of specimens by using a new specimen collection container for each specimen obtained.
- Read the entire procedure carefully prior to performing any tests.
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