Vitamin D VD Rapid Test Kit 25 (OH) D in whole blood, serum and plasma
INTENDED USE
The Vitamin D Rapid Test Cassette is a rapid chromatographic immunoassay for the semi-quantitative detection of 25-hydroxyvitamin D (25 (OH) D) in human whole blood, serum and plasma. This assay provides a preliminary diagnostic test result and can be used to screening for Vitamin D deficiency.
For professional use only.
For in vitro diagnostic use only.
Storage |
2~30 º C |
Specimen |
Whole Blood/Serum/Plasma |
Component |
Rapid Test +Extraction Buffer + Pipettes |
Principle |
Colloidal gold rapid tests |
Reading |
Within 15mins |
Package |
40tests/box; 25tests/box |
Trademark |
Dewei |
Origin |
China |
INTRODUCTION
Vitamin D refers to a group of fat-soluble secosteroids responsible for increasing intestinal absorption of calcium, iron, magnesium, phosphate and zinc. In humans, the most important compounds in this group are vitamin D3 and vitamin D2. Vitamin D3 is naturally produced in the human skin through the exposure to ultraviolet light and Vitamin D2 is mainly obtained from foods. Vitamin D is transported to the liver where it is metabolized to 25-hydroxy Vitamin D. In medicine, a 25-hydroxy Vitamin D blood test is used to determine Vitamin D concentration in the body. The blood concentration of 25-hydroxy Vitamin D (including D2 and D3) is considered the best indicator of Vitamin D status. Vitamin D deficiency is now recognized as a global epidemic. Virtually every cell in our body has Receptors for Vitamin D, meaning that they all require “Sufficient” Level of Vitamin D for adequate functioning. The health risks associated with Vitamin D deficiency are far more severe than previously thought. Vitamin deficiency has been linked to various serious diseases: Osteoporosis, Osteomalacia, Multiple Sclerosis, Cardiovascular Diseases, Pregnancy Complications, Diabetes, Depression, Strokes, Autoimmune Diseases, Flu, Different Cancers, Infectious Diseases, Alzheimer, Obesity and Higher Mortality etc. Therefore, now detecting (25-OH) Vitamin D level is considered as “Medically Necessary Screening Test”, and maintaining sufficient levels not just to improve bone health, but to improve overall health and well-being.
MAIN CONTENTS
Rapid Test Cassette in Pouch
Extraction Buffer
Disposable dropper
Package insert
SRORAGE
Store as packaged in the sealed pouch at room temperature or refrigerated (2-30°C).
The test is stable through the expiration date printed on the sealed pouch.
The test must remain in the sealed pouch until use.
DO NOT FREEZE.
Do not use beyond the expiration date.
OPERATION PROCEDURE
Bring tests, specimens, buffer and/or controls to room temperature (15-30°C) before use.
1.Add 4 drops of buffer into Extraction tube, and transfer 1 drop of whole blood, serum or plasma sample (to the Fill Line as shown in illustration below) into tube.
2.Gently mixing the sample and buffer for 15minutes.
3.Transfer 2 drops of mixing sample into specimen well of the test cassette, then start the timer. Avoid trapping air bubbles in the specimen well.
4.Read results at 15 minutes. Don’t interpret result after 20 minutes.
FRQ:
1) What exactly does vitamin D do for you?
Vitamin D is a nutrient your body needs for building and maintaining healthy bones. That's because your body can only absorb calcium, the primary component of bone, when vitamin D is present. Vitamin D also regulates many other cellular functions in your body.
2) What are signs of low vitamin D?
What are the symptoms of vitamin D deficiency?
Muscle pain.
Bone pain.
Increased sensitivity to pain.
A tingly, “pins-and-needles” sensation in the hands or feet.
Muscle weakness in body parts near the trunk of the body, such as the upper arms or thighs.
Waddling while walking, due to muscle weakness in the hips or legs.
3) How can I raise my vitamin D level quickly?
Here are three ways to increase your intake:
Eat foods high in vitamin D, like fish or fortified breakfast foods (milk, cereals and orange juice).
Get vitamin D from moderate sunlight exposure each day.
Take a vitamin D3 supplement or cod liver oil.
4) How to gain vitamin D?
Good sources of vitamin D
oily fish – such as salmon, sardines, herring and mackerel.
red meat.
liver (avoid liver if you are pregnant)
egg yolks.
fortified foods – such as some fat spreads and breakfast cereals.
5) How long does it take to correct vitamin D levels?
“Adding an over-the-counter vitamin D supplement can make improvements in just three to four months. Vitamin D with a strength of 1000-2000 international units daily is the recommended dose for most adults,” Dr. Ropte says. Most multivitamins contain vitamin D, so extra supplementation isn't always necessary.
6) How much vitamin D per day to raise levels?
The Endocrine Society states, for example, that to maintain serum 25(OH)D levels above 75 nmol/L (30 ng/mL), adults might need at least 37.5 to 50 mcg (1,500–2,000 IU)/day of supplemental vitamin D, and children and adolescents might need at least 25 mcg (1,000 IU)/day
7) Is 2 eggs a day enough vitamin D?
With a serve of two eggs providing 82% of your daily recommended intake of vitamin D, eggs contain one of the highest quantities of vitamin D of any food. Here, Australian Eggs has delved into what exactly vitamin D is, how it benefits our bodies, and where you can find this important nutrient in your daily diet.
8) What food is highest in vitamin D?
The best food sources of vitamin D are oily fish, including salmon, mackerel, and sardines. Other sources include egg yolks, red meat, and liver. Vitamin D is added to some foods too, including breakfast cereals, plant milks and fat spreads. Check information on-pack to see if this applies to the products you buy.
9) What depletes vitamin D?
You can become deficient in vitamin D for different reasons: You don't get enough vitamin D in your diet. You don't absorb enough vitamin D from food (a malabsorption problem) You don't get enough exposure to sunlight.
10) When to take vitamin D, morning or night?
Try taking it alongside breakfast or with a bedtime snack — as long as it doesn't interfere with your sleep. The key is to find what works for you and stick with it to ensure you're meeting your vitamin D needs. Taking vitamin D with a meal can increase its absorption, but studies on specific timing are limited.
11) Is it safe to take 5000 IU of vitamin D3 every day?
Some people may need a higher dose, however, including those with a bone health disorder and those with a condition that interferes with the absorption of vitamin D or calcium, says Dr. Manson. Unless your doctor recommends it, avoid taking more than 4,000 IU per day, which is considered the safe upper limit.
12) Is it better to take vitamin D every day or once a week?
Experts advise adults aged 19–70 to regularly take 15 micrograms (mcg), or 600 international units (IU), of vitamin D per day.
13) What happens if I take too much vitamin D?
The main consequence of vitamin D toxicity is a buildup of calcium in your blood (hypercalcemia), which can cause nausea and vomiting, weakness, and frequent urination. Vitamin D toxicity might progress to bone pain and kidney problems, such as the formation of calcium stones.
14) How soon will I feel better after taking vitamin D3?
In general, some individuals may start to notice improvements in their Vitamin D levels within a few weeks of starting supplementation, while for others, it may take a bit longer. It's essential to be patient and consistent with your supplement regimen to allow your body to absorb and utilize Vitamin D effectively.
For details, please contact Dewei staff for Instruction Manual!

