CE Dengue Antigen NS1 IGG IGM Rapid Test Kit Cassette Combo Duo Diagnostic
【INTENDED USE】
The Dengue NS1 & IgG/IgM Rapid Test Combo is a rapidchromatographic immunoassay for the qualitative detection of dengue
virus NS1 antigen, IgG and IgM antobody in human whole blood,serum, or plasma as an aid in the diagnosis of primary and secondary Dengue infections.
Serum and Plasma sample is better recommended for testing.
【INTRODUCTION】
Dengue viruses, transmitted by the mosquito, Aedes aegypti and Aedes albopictus mosquitoes, are widely distributed throughout the tropical and subtropical areas of the world. There are four known distinct serotypes (dengue virus 1, 2, 3 and 4). In children, infection is often subclinical or causes a self-limited febrile disease. However, if the patient is infected second times with a different serotype, a more severe disease, dengue hemorrhagic fever or dengue shock syndrome, is more likely to occur. Dengue is considered to be the most important arthropod-borne viral disease due to the human morbidity and mortality it causes. NS1 is a highly-conserved glycoprotein thatis present at high concentrations in the sera of dengue-infected patients during the early clinical phase of the disease. NS1 antigen is found from the first day and up to 9 days after onset of fever in sample of primary or secondary dengue infected patients.
【SPECIMEN COLLECTION AND PREPARATION】
The Dengue NS1 & IgG/IgM Rapid Test Combo is intended for use with human whole blood, serum or plasma specimens only.
Only clear, non-hemolyzed specimens are recommended for use with this test. Serum or plasma should be separated as soon as possible to avoid hemolysis.
Perform testing immediately after specimen collection. Do not leave specimens at room temperature for prolonged periods. Serum and plasma specimens may be stored at 2-8°C for up to 3 days. For long term storage, specimens should be kept below -20°C. Whole blood collected by venipuncture should be stored at 2-8°C if the test is to be run within 2 days of collection. Do not
freeze whole blood specimens. Whole blood collected by fingerstick should be tested immediately.
Containers containing anticoagulants such as EDTA, citrate, or heparin should be used for whole blood storage.
Bring specimens to room temperature prior to testing. Frozen specimens must be completely thawed and mixed well prior to testing. Avoid repeated freezing and thawing of specimens.
If specimens are to be shipped, pack them in compliance with all applicable regulations for transportation of etiological agents.
Icteric, lipemic, hemolysed, heat treated and contaminated seram may cause erroneous results.
【MATERIALS 】
- Test cassettes
- Droppers
- Buffer
- Package insert
Materials required but not provided
- Specimen collection containers
- Centrifuge (for plasma only)
- Micropipette
- Timer
【DIRECTIONS FOR USE】
1.Bring tests, specimens, buffer and/or controls to room temperature (15- 30°C) before use.
2.Remove the test from its sealed pouch, and place it on a clean, level surface. Label the device with patient or control identification. For best results, the assay should be performed within one hour.
NS1 Antigen:
Using the provided disposable pipette, transfer 2 drops of Serum or Plasma specimen into the center of the specimen well(s) of the tes cassette.
OR
Transfer 1 drop of Whole Blood specimen into the center of the specimen well(s) of the test cassette, then add 1 drop of buffer, and start the timer.
IgG/IgM Antibody:
Transfer 1 drop of sample into the center of the specimen well(s) of the test cassette, then add 1 drop of buffer, and start the timer.
3.Wait for the colored band(s) to appear. The result should be read at 15 minutes. Do not interpret the result after 20 minutes.
【INTERPRETATION OF RESULTS】
NS1 POSITIVE: Two colored bands appear on the membrane. One band appears in the control region (C) and another band appears in the test region (T).
NS1 NEGATIVE: Only one colored band appears, in the control region (C). No apparent colored band appears in the test region (T).
INVALID: Control band fails to appear. Results from any test which has not produced a control band at the specified read time must be discarded. Please review the procedure and repeat with a new test. If the problem persists, discontinue using the kit immediately and contact your local distributor.
IgM POSITIVE:* The red line in the control line region (C) , and a colored line appears in test line IgM region. The result is positive for Dengue virus specific-IgM antibodies.
IgG POSITIVE:* The red line in the control line region (C), and a red line appears in test line IgG antibodies. The result is positive for Dengue virus specific-IgG antibodies.
IgG and IgM POSITIVE:* The red line in the control line region (C) and two red lines should appear in test line IgM and IgG regions. The color intensities of the lines do not have to match. The result is positive for IgG & IgM antibodies.
FRQ:
| 1 |
What are 7 warning signs of dengue? |
Warning Signs*
Abdominal pain or tenderness.
Persistent vomiting.
Clinical fluid accumulation.
Mucosal bleed.
Lethargy or restlessness.
Liver enlargement > 2 cm.
Laboratory finding of increasing HCT concurrent with rapid decrease in platelet count. |
| 2 |
How long will dengue fever last? |
How Long Does Dengue Fever Last? Symptoms can start anywhere from 4 days to 2 weeks after being bitten by an infected mosquito, and typically last for 2 to 7 days. |
| 3 |
What is the best treatment for dengue? |
Most cases of dengue fever can be treated at home with pain medicine. Acetaminophenparacetamol is often used to control pain. Non-steroidal anti-inflammatory drugs like ibuprofen and aspirin are avoided as they can increase the risk of bleeding. For people with severe dengue, hospitalization is often needed. |
| 4 |
Is dengue very serious? |
Severe dengue fever can cause internal bleeding and organ damage. Blood pressure can drop to dangerous levels, causing shock. In some cases, severe dengue fever can lead to death. Women who get dengue fever during pregnancy may be able to spread the virus to the baby during childbirth. |
| 5 |
How do you confirm dengue? |
If an infection is suspected, you will get a blood test to check for the dengue virus. During a blood test, a health care professional will take a blood sample from a vein in your arm, using a small needle. After the needle is inserted, a small amount of blood will be collected into a test tube or vial. |
| 6 |
What not to eat in dengue? |
It is best to choose a lighter diet and avoid oily and fried food. It contains a lot of fat which may lead to higher blood pressure. This can hamper your recovery as it weakens the immune system. |
| 7 |
What is the danger period of dengue? |
Dengue in the critical phase occurs from the 4th to the 7th day since the patient shows symptoms of fever. At this time, the patient may not have a fever or reduce his fever, so he will appear subjective and do not go to the hospital for treatment, making the disease more dangerous. |
| 8 |
Can dengue recover by itself? |
Dengue fever usually lasts between 2 to 7 days.
Most people recover from dengue infection without requiring hospital admission. |
| 9 |
What kills dengue virus? |
To fight the infection, the immune system produces antibodies to neutralize the dengue viral particles, and the complement system is activated to help the antibodies and white blood cells remove the virus. The immune response also includes cytotoxic T cells (lymphocytes), which recognize and kill infected cells. |
| 10 |
Which antibiotic is best for dengue? |
There is no specific medicine to treat dengue infection. If you think you may have dengue fever, you should use pain relievers withacetaminophen and avoid medicines with aspirin, which could worsen bleeding. You should also rest, drink plenty of fluids, and see your doctor. |
| 11 |
How to recover fast from dengue? |
Turmeric with milk is best remedy for faster recovery. Citrus fruits play an important role for dengue patients and it help in recovery. So, rich mix of antioxidants and vitamin C like orange, strawberries, lemon, papaya etc should be included in a diet twice a day," says Dr Sharma. |
| 12 |
Is a CBC test for dengue? |
Complete Blood Count (CBC): The complete blood count test is employed to assess various components of the blood. One crucial aspect is the platelet count, which tends to decrease during the later stages of dengue fever. Monitoring platelet levels is essential as low platelet count can lead to bleeding complications. |
| 13 |
How many days do platelets fall in dengue? |
Platelet counts usually drop significantly by day 4 of illness. In adults without dengue shock, platelet counts drop slightly to moderate from day 3 to day 7 of illness and return to normal by day 8 or 9. |
| 14 |
What is the recovery stage of dengue? |
The recovery phase is the final stage of dengue fever. Individuals who have passed the febrile phase without entering the critical phase, or those who have recovered from the critical second phase for 1-2 days, will reach the recovery phase. |
| 15 |
Can I test dengue at home? |
These tests look for the NS1 protein from the dengue virus in the blood. They usually use special antibodies with labels to find this protein. Orange Health Labs offers the convenience of dengue fever NS1 Antigen Rapid Test at home anywhere in Bangalore. |
| 16 |
Can I drink milk in dengue? |
You can drink more milk to enhance nutrition. Do not eat rice or hard foods that require a lot of chewing and are difficult to swallow. Especially, for children with dengue fever, milk is the main and most important source of nutrition. |
| 17 |
What to not eat in dengue? |
Dengue patients must exclude raw & strongly flavored veggies from their diet, such as cabbage, capsicum, turnip, radish, onion, garlic, eggplant, etc.
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